Article
Single-cell multi-omics defines the cell-type-specific impact of splicing aberrations in human hematopoietic clonal outgrowths.
Cell stem cell - 7 Sept 2023
Cortés-López Mariela, Chamely Paulina, Hawkins Allegra G, Stanley Robert F, Swett Ariel D, Ganesan Saravanan, Mouhieddine Tarek H, Dai Xiaoguang, Kluegel Lloyd, Chen Celine, Batta Kiran, Furer Nili, Vedula Rahul S, Beaulaurier John, Drong Alexander W, Hickey Scott, Dusaj Neville, Mullokandov Gavriel, Stasiw Adam M, Su Jiayu, Chaligné Ronan, Juul Sissel, Harrington Eoghan, Knowles David A, Potenski Catherine J, Wiseman Daniel H, Tanay Amos, Shlush Liran, Lindsley Robert C, Ghobrial Irene M, Taylor Justin, Abdel-Wahab Omar, Gaiti Federico, Landau Dan A
Abstract excerpt
RNA splicing factors are recurrently mutated in clonal blood disorders, but the impact of dysregulated splicing in hematopoiesis remains unclear. To overcome technical limitations, we integrated genotyping of transcriptomes (GoT) with long-read single-cell transcriptomics and proteogenomics for single-cell profiling of transcriptomes, surface proteins, somatic mutations, and RNA splicing (GoT-Splice). We applied...
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