Article
Efficient infection by a recombinant Kaposi's sarcoma-associated herpesvirus cloned in a bacterial artificial chromosome: application for genetic analysis.
Journal of virology - 1 Jun 2002
Zhou Fu-Chun, Zhang Yan-Jin, Deng Jian-Hong, Wang Xin-Ping, Pan Hong-Yi, Hettler Evelyn, Gao Shou-Jiang
Abstract excerpt
Kaposi's sarcoma-associated herpesvirus (KSHV) is etiologically associated with Kaposi's sarcoma and several other malignancies. The lack of an efficient infection system has impeded the understanding of KSHV-related pathogenesis. A genetic approach was used to isolate infectious KSHV. Recombinant bacteria artificial chromosome (BAC) KSHV containing hygromycin resistance and green fluorescent protein (GFP)...
Topics
- Cell Line
- Chromosomes, Artificial, Bacterial
- Cloning, Molecular
- Escherichia coli
- Genome, Viral
- HeLa Cells
- Herpesviridae Infections
- Herpesvirus 8, Human
- Humans
- Mutation
- Recombination, Genetic
- Transfection
- Tumor Cells, Cultured