Endonuclease IV and T4 ligase enhanced detection of mutations in low abundance.

Abstract excerpt

We propose a mutant detection approach based on endonuclease IV and DNA ligase in combination with qPCR. The enzymes functioned cooperatively to facilitate PCR for low abundance DNA detection. We demonstrate that our approach can distinguish mutations as low as 0.01%, indicating the potential application of this strategy in early cancer diagnosis.

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• Share to Deoxyribonuclease IV (Phage T4-Induced)
• Share to DNA
• Share to DNA Ligases