Article
Analysis of the Plasmid-Based ts Allele of PA0006 Reveals Its Function in Regulation of Cell Morphology and Biosynthesis of Core Lipopolysaccharide in Pseudomonas aeruginosa.
Applied and environmental microbiology - 26 Jul 2022
Yang Zhili, Zhang Zengping, Zhu Jiaqi, Ma Yunhao, Wang Jianxin, Liu Jianhua
Abstract excerpt
Over 300 essential genes are predicted using transposon sequencing in the genome of Pseudomonas aeruginosa. However, methods for reverse genetic analysis of essential genes are scarce. To address this issue, we developed a three-step protocol consisting of integration of deletion plasmid, introduction of temperature-sensitive rescue plasmid, and excision of integrated-deletion plasmid to construct the...
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