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Article

Quantitative analysis of single-nucleotide polymorphisms by pyrosequencing with di-base addition.

Electrophoresis - 1 Mar 2017

Pu Dan, Pan Rongfang, Liu Wenbin, Xiao Pengfeng

Abstract excerpt

We have developed and validated a novel method for quantitative detection of SNPs by using pyrosequencing with di-base addition (PDBA). Based on the principle that the signal intensity is proportional to the template concentration within a linear concentration range, linear formula (Y = AX + B) for each genotype is established, and the relationship between two genotypes of a single SNP can be resolved by...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 27957738
DOI: 10.1002/elps.201600430

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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