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Article

Epigenetic profiles guide improved CRISPR/Cas9-mediated gene knockout in human T cells

21 Nov 2023

Abstract excerpt

Genetic modification of specific genes is emerging as a useful tool to enhance the functions of antitumor T cells in adoptive immunotherapy. Current advances in CRISPR/Cas9 technology enable gene knockout during in vitro preparation of infused T-cell products through transient transfection of a Cas9-guide RNA (gRNA) ribonucleoprotein complex. However, selecting optimal gRNAs remains a major challenge for...

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PMID: 37985205
DOI: 10.1093/nar/gkad1076

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