Article
RatioCRISPR: A ratiometric biochip based on CRISPR/Cas12a for automated and multiplexed detection of heteroplasmic SNPs in mitochondrial DNA.
Biosensors & bioelectronics - 1 Dec 2023
Wu Xiaolong, Zhao Yi, Guo Chuanghao, Liu Conghui, Zhang Qianling, Chen Yong, Liu Yizhen, Zhang Xueji
Abstract excerpt
Mitochondrial genetic diseases are often characterized by heteroplasmic single nucleotide polymorphisms (SNPs) where both wild-type (WT) and mutant-type (MT) coexist, making detection of accurate SNP abundance critical for diagnosis. Here, we present RatioCRISPR, an automated ratiometric biochip sensor based on the CRISPR/Cas12a system for detecting multiple heteroplasmic SNPs in mitochondrial DNA (mtDNA). The...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.