Article
Generation and application of endogenously floxed alleles for cell-specific knockout in zebrafish.
Developmental cell - 20 Nov 2023
Shin Masahiro, Yin Hui-Min, Shih Yu-Huan, Nozaki Takayuki, Portman Daneal, Toles Benjamin, Kolb Amy, Luk Kevin, Isogai Sumio, Ishida Kinji, Hanasaka Tomohito, Parsons Michael J, Wolfe Scot A, Burns Caroline E, Burns C Geoffrey, Lawson Nathan D
Abstract excerpt
The zebrafish is amenable to a variety of genetic approaches. However, lack of conditional deletion alleles limits stage- or cell-specific gene knockout. Here, we applied an existing protocol to establish a floxed allele for gata2a but failed to do so due to off-target integration and incomplete knockin. To address these problems, we applied simultaneous co-targeting with Cas12a to insert loxP sites in cis,...
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