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Article

Bi-FoRe: an efficient bidirectional knockin strategy to generate pairwise conditional alleles with fluorescent indicators.

Protein & cell - 1 Jan 2021

Han Bingzhou, Zhang Yage, Bi Xuetong, Zhou Yang, Krueger Christopher J, Hu Xinli, Zhu Zuoyan, Tong Xiangjun, Zhang Bo

Abstract excerpt

Gene expression labeling and conditional manipulation of gene function are important for elaborate dissection of gene function. However, contemporary generation of pairwise dual-function knockin alleles to achieve both conditional and geno-tagging effects with a single donor has not been reported. Here we first developed a strategy based on a flipping donor named FoRe to generate conditional knockout alleles...

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Identifiers

PMID: 32681448
DOI: 10.1007/s13238-020-00747-1
PMCID: PMC7815861

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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