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Article

Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging.

STAR protocols - 15 Sept 2023

Zilliox Marie, Tillement Vanessa, Mangeat Thomas, Polès Sophie, Blader Patrick, Batut Julie

Abstract excerpt

Temporal and spatial regulation of gene expression is crucial for proper embryonic development. Infrared laser-evoked gene operator (IR-LEGO) can provide information for various developmental processes. Here, we present a protocol to locally express cxcl12a during zebrafish olfactory organ development1 using a combination of IR-LEGO and live imaging. We describe steps for implementing IR-LEGO, biological sample...

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Identifiers

PMID: 37624700
DOI: 10.1016/j.xpro.2023.102538
PMCID: PMC10463262

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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