Article
Accelerated secretion of mutant beta-lactoglobulin in Saccharomyces cerevisiae resulting from a single amino acid substitution.
Biochimica et biophysica acta - 13 Jul 1999
Katakura Y, Ametani A, Totsuka M, Nagafuchi S, Kaminogawa S
Abstract excerpt
Transformed yeasts producing a mutant form of bovine beta-lactoglobulin (beta-LG), W19Y, in which Trp(19) was replaced with Tyr, were shown to secrete 6 times more than those producing wild type beta-LG. Northern blot analysis suggested that the enhanced level of secretion was not the result of upregulated transcription of W19Y. The ratio of the amount of W19Y secreted into the supernatant to the amount of W19Y...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.