Article
Mutational and structural analyses of UdgX: insights into the active site pocket architecture and its evolution.
Nucleic acids research - 21 Jul 2023
Aroli Shashanka, Woo Eui-Jeon, Gopal Balasubramanian, Varshney Umesh
Abstract excerpt
UdgX excises uracil from uracil-containing DNA to concurrently form a covalent bond with the resulting AP-DNA. Structurally, UdgX is highly similar to family-4 UDGs (F4-UDGs). However, UdgX is unique in possessing a flexible R-loop (105KRRIH109). Among the class-defining motifs, while its motif A (51GEQPG55) diverged to possess Q53 in place of A53/G53 in F4-UDGs, motif B [178HPS(S/A)(L/V)(L/V)R184] has remained...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.