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Article

Substitutions at tyrosine 66 of Escherichia coli uracil DNA glycosylase lead to characterization of an efficient enzyme that is recalcitrant to product inhibition.

Nucleic acids research - 15 Dec 2003

Acharya Narottam, Talawar Ramappa K, Saikrishnan K, Vijayan M, Varshney Umesh

Abstract excerpt

Uracil DNA glycosylase (UDG), a ubiquitous and highly specific enzyme, commences the uracil excision repair pathway. Structural studies have shown that the tyrosine in a highly conserved GQDPY water-activating loop of UDGs blocks the entry of thymine or purines into the active site pocket. To further understand the role of this tyrosine (Y66 in Escherichia coli UDG), we have overproduced and characterized Y66F,...

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Identifiers

PMID: 14654697
DOI: 10.1093/nar/gkg918
PMCID: PMC291862

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