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Article

Photoinduced proton transfer inside an engineered green fluorescent protein: a stepwise-concerted-hybrid reaction.

Physical chemistry chemical physics : PCCP - 9 May 2018

Tang Longteng, Wang Yanli, Zhu Liangdong, Kallio Karen, Remington S James, Fang Chong

Abstract excerpt

Photoactivated proton transfer (PT) wire is responsible for the glow of green fluorescent protein (GFP), which is crucial for bioimaging and biomedicine. In this work, a new GFP-S65T/S205V double mutant is developed from wild-type GFP in which the PT wire is significantly modified. We implement femtosecond transient absorption (fs-TA) and femtosecond stimulated Raman spectroscopy (FSRS) to delineate the PT...

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PMID: 29708241
DOI: 10.1039/c8cp01907j

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