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Article

Quantification of Isoniazid-Heteroresistant Mycobacterium tuberculosis Using Droplet Digital PCR.

Journal of clinical microbiology - 20 Jun 2023

Zhang Siqi, Chen Xiaohong, Lin Zhonghui, Tan Yaoju, Liang Bin, Pan Yuying, Huang Mingxiang, Su Biyi, Hu Xiaoman, Xu Ye, Li Qingge

Abstract excerpt

The quantitative detection of drug-resistance mutations in Mycobacterium tuberculosis (MTB) is critical for determining the drug resistance status of a sample. We developed a drop-off droplet digital PCR (ddPCR) assay targeting all major isoniazid (INH)-resistant mutations. The ddPCR assay consisted of three reactions: reaction A detects mutations at katG S315; reaction B detects inhA promoter mutations; and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 37195177
DOI: 10.1128/jcm.01884-22
PMCID: PMC10281145

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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