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Article

Improved detection of isoniazid-heteroresistant Mycobacterium tuberculosis subpopulations by droplet digital PCR compared to MeltPro TB assay.

Microbiology spectrum - 7 Oct 2025

Li Haoran, Wang Nan, Yang Mengjie, Shang Yuanyuan, Wang Yilin, Tan Chianru, Wen Shufang, Guo Yong, Pang Yu

Abstract excerpt

Drug resistance in Mycobacterium tuberculosis (Mtb), especially isoniazid (INH) resistance, challenges tuberculosis control. This study evaluated droplet digital PCR (ddPCR) against the traditional MeltPro TB assay. A total of 77 INH-resistant samples from Beijing Chest Hospital, China, underwent ddPCR, drug susceptibility testing, and Sanger sequencing. Of the 73 valid samples, MeltPro detected 55 INH-resistant...

Read the complete abstract on PubMed

Topics

Isoniazid
Mycobacterium tuberculosis
Humans
Antitubercular Agents
Microbial Sensitivity Tests
Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Drug Resistance, Bacterial

Tuberculosis, Multidrug-Resistant

Mutation

Sensitivity and Specificity

Tuberculosis

China

Source

Open in PubMed Open full text

Identifiers

PMID: 40856496
DOI: 10.1128/spectrum.00030-25
PMCID: PMC12502712

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