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Article

Identification of residues in the first transmembrane domain of the P2X7 that regulates receptor trafficking, sensitization, and dye uptake function.

Journal of neurochemistry - 1 Jun 2023

Rupert Marian, Bhattacharya Anirban, Sivcev Sonja, Knezu Michal, Cimicka Jana, Zemkova Hana

Abstract excerpt

P2X receptors (P2X1-7) are trimeric ion channels activated by extracellular ATP. Each P2X subunit contains two transmembrane helices (TM1 and TM2). We substituted all residues in TM1 of rat P2X7 with alanine or leucine one by one, expressed mutants in HEK293T cells, and examined the pore permeability by recording both membrane currents and fluorescent dye uptake in response to agonist application. Alanine...

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PMID: 36945903
DOI: 10.1111/jnc.15813

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Identification of residues in the first transmembrane domain of the P2X7 that regulates receptor trafficking, sensitization, and dye uptake function.PMID 36945903Read ↗

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