Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Deciphering integration loci of CHO manufacturing cell lines using long read nanopore sequencing

14 Mar 2023

Abstract excerpt

Despite advances in genetic characterization of Chinese hamster ovary (CHO) cell lines regarding identification of integration sites using next generation sequencing, e.g. targeted locus amplification sequencing (TLA-seq), the concatemer structure of the integrated vectors remains elusive. Here, the entire integration locus of two CHO manufacturing cell lines was reconstructed combining CRISPR/Cas9 target...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR and Genetic Engineering
Share to Monoclonal and Polyclonal Antibodies Research
Share to Viral Infectious Diseases and Gene Expression in Insects

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 36925062
DOI: 10.1016/j.nbt.2023.03.003

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Deciphering integration loci of CHO manufacturing cell lines using long read nanopore sequencingPMID 36925062Read ↗

Structural analysis of random transgene integration in CHO manufacturing cell lines by targeted sequencingSemantic proximity Read ↗

Minimizing Clonal Variation during Mammalian Cell Line Engineering for Improved Systems Biology Data GenerationSemantic proximity Read ↗

CRISPR‐interceded CHO cell line development approachesSemantic proximity Read ↗

Baculovirus-vectored precision delivery of large DNA cargoes in human genomesSemantic proximity Read ↗

Analysis of polyclonal vector integration sites using Nanopore sequencing as a scalable, cost-effective platformSemantic proximity Read ↗

Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor PlasmidsSemantic proximity Read ↗

GREPore-seq: A Robust Workflow to Detect Changes after Gene Editing through Long-range PCR and Nanopore SequencingSemantic proximity Read ↗

Systematic Evaluation of Site-Specific Recombinant Gene Expression for Programmable Mammalian Cell EngineeringSemantic proximity Read ↗

Efficient nonviral integration of large transgenes into human T cells using Cas9-CLIPTSemantic proximity Read ↗

Target-enriched nanopore sequencing and de novo assembly reveals co-occurrences of complex on-target genomic rearrangements induced by CRISPR-Cas9 in human cells.Semantic proximity Read ↗

A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian CellsSemantic proximity Read ↗

INSERT-seq enables high resolution mapping of genomically integrated DNA using nanopore sequencingSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.