Article

Analysis of polyclonal vector integration sites using Nanopore sequencing as a scalable, cost-effective platform

2019-11-07

Abstract excerpt

Vector integration site analysis can be important in the follow-up of patients who received gene-modified cells, but current platforms based on next-generation sequencing are expensive and relatively inaccessible. We analyzed polyclonal T cells transduced by a gammaretroviral vector, SFG.iCasp9.2A.ΔCD19, from a clinical trial. Following restriction enzyme digestion, the unknown flanking genomic sequences were ampl...

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Literature Corpus work: 5e32f619-f0d7-5409-8127-9b8dd6551e85
DOI: 10.1101/833897

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