Article
Limitations of gene editing assessments in human preimplantation embryos.
Nature communications - 7 Mar 2023
Liang Dan, Mikhalchenko Aleksei, Ma Hong, Marti Gutierrez Nuria, Chen Tailai, Lee Yeonmi, Park Sang-Wook, Tippner-Hedges Rebecca, Koski Amy, Darby Hayley, Li Ying, Van Dyken Crystal, Zhao Han, Wu Keliang, Zhang Jingye, Hou Zhenzhen, So Seongjun, Han Jongsuk, Park Jumi, Kim Chong-Jai, Zong Kai, Gong Jianhui, Yuan Yilin, Gu Ying, Shen Yue, Olson Susan B, Yang Hui, Battaglia David, O'Leary Thomas, Krieg Sacha A, Lee David M, Wu Diana H, Duell P Barton, Kaul Sanjiv, Kim Jin-Soo, Heitner Stephen B, Kang Eunju, Chen Zi-Jiang, Amato Paula, Mitalipov Shoukhrat
Abstract excerpt
Range of DNA repair in response to double-strand breaks induced in human preimplantation embryos remains uncertain due to the complexity of analyzing single- or few-cell samples. Sequencing of such minute DNA input requires a whole genome amplification that can introduce artifacts, including coverage nonuniformity, amplification biases, and allelic dropouts at the target site. We show here that, on average, 26.6%...
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