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Article

Sequence terminus dependent PCR for site-specific mutation and modification detection.

Nature communications - 1 Mar 2023

Xu Gaolian, Yang Hao, Qiu Jiani, Reboud Julien, Zhen Linqing, Ren Wei, Xu Hong, Cooper Jonathan M, Gu Hongchen

Abstract excerpt

The detection of changes in nucleic acid sequences at specific sites remains a critical challenge in epigenetics, diagnostics and therapeutics. To date, such assays often require extensive time, expertise and infrastructure for their implementation, limiting their application in clinical settings. Here we demonstrate a generalizable method, named Specific Terminal Mediated Polymerase Chain Reaction (STEM-PCR) for...

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PMID: 36859350
DOI: 10.1038/s41467-023-36884-4
PMCID: PMC9978023

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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