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Article

A polymerase engineered for bisulfite sequencing.

Nucleic acids research - 15 Dec 2015

Millar Doug, Christova Yonka, Holliger Philipp

Abstract excerpt

Bisulfite sequencing is a key methodology in epigenetics. However, the standard workflow of bisulfite sequencing involves heat and strongly basic conditions to convert the intermediary product 5,6-dihydrouridine-6-sulfonate (dhU6S) (generated by reaction of bisulfite with deoxycytidine (dC)) to uracil (dU). These harsh conditions generally lead to sample loss and DNA damage while milder conditions may result in...

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Identifiers

PMID: 26271989
DOI: 10.1093/nar/gkv798
PMCID: PMC4678845

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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