Article
Amplifluor-Based SNP Genotyping.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Mohanrao Manmode Darpan, Senthilvel Senapathy, Reddy Yarabapani Rushwanth, Kumar Chippa Anil, Kadirvel Palchamy
Abstract excerpt
Amplifluor, a genotyping system used to analyze single nucleotide polymorphisms (SNPs), is supplied by Merck-Millipore. Amplifluor is based on polymerase chain reaction (PCR) with two competing allele-specific primers and a SNP specific common reverse primer. Sequence information flanking SNP of interest and fluorescent plate reader for end-point measurement or qPCR machine for real time measurement are required...
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