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Article

Genotyping using the TaqMan assay.

Current protocols in human genetics - 1 Jan 2008

Hui Lester, DelMonte Terrye, Ranade Koustubh

Abstract excerpt

The 5'-nuclease allelic discrimination assay, or TaqMan assay, is a PCR-based assay for genotyping single nucleotide polymorphisms (SNPs). The region flanking the SNP is amplified in the presence of two allele-specific fluorescent probes. The probes do not fluoresce in solution because of a quencher at the 3' end. The presence of two probes allows the detection of both alleles in a single tube. Moreover, because...

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PMID: 18428424
DOI: 10.1002/0471142905.hg0210s56

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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