Article
SNP Genotyping with Amplifluor-Like Method.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Khassanova Gulmira, Khalbayeva Sholpan, Serikbay Dauren, Mazkirat Shynar, Bulatova Kulpash, Utebayev Maral, Shavrukov Yuri
Abstract excerpt
For SNP genotyping, amplification of fluorescence (Amplifluor) is a popular and actively developing method in the plant sciences. The "Amplifluor-like" is a "home-made" modification of the original commercial Amplifluor method. Amplifluor-like genotyping requires two essential components: (1) two allele-specific forward primers targeting the SNP site with one common reverse primer; and (2) a universal part with...
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