Article
Simultaneous detection of JAK2, CALR, and MPL mutations and quantitation of JAK2 V617F allele burden in myeloproliferative neoplasms using the quenching probe-Tm method in i-densy IS-5320.
International journal of laboratory hematology - 1 Dec 2022
Arai Kunihito, Sakaguchi Masahiro, Yui Shunsuke, Kitano Tomoaki, Miyata Miho, Yogosawa Mayumi, Nakayama Kazutaka, Tajika Kenji, Usuki Kensuke, Kuroda Junya, Uoshima Nobuhiko, Kobayashi Yutaka, Uchiyama Hitoji, Kubota Yasushi, Kimura Shinya, Mori Shinichiro, Hirai Mitsuharu, Wakita Satoshi, Yamaguchi Hiroki
Abstract excerpt
INTRODUCTION: Accurate detection of myeloproliferative neoplasms (MPN)-associated gene mutations is necessary to correctly diagnose MPN. However, conventional gene testing has various limitations, including the requirement of skilled technicians, cumbersome experimental procedures, and turnaround time of several days. The gene analyzer i-densy IS-5320 allows gene testing using the quenching probe-Tm method....
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