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Article

Genetic Perturbation Alters Functional Substates in Alkaline Phosphatase.

Journal of the American Chemical Society - 8 Feb 2023

Sakuma Morito, Honda Shingo, Ueno Hiroshi, Tabata Kazuhito V, Miyazaki Kentaro, Tokuriki Nobuhiko, Noji Hiroyuki

Abstract excerpt

Enzymes inherently exhibit molecule-to-molecule heterogeneity in their conformational and functional states, which is considered to be a key to the evolution of new functions. Single-molecule enzyme assays enable us to directly observe such multiple functional states or functional substates. Here, we quantitatively analyzed functional substates in the wild-type and 69 single-point mutants of Escherichia coli...

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Identifiers

PMID: 36706363
DOI: 10.1021/jacs.2c06693
PMCID: PMC9912328

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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