Article
A ten-week biochemistry lab project studying wild-type and mutant bacterial alkaline phosphatase.
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology - 12 Nov 2016
Witherow D Scott
Abstract excerpt
This work describes a 10-week laboratory project studying wild-type and mutant bacterial alkaline phosphatase, in which students purify, quantitate, and perform kinetic assays on wild-type and selected mutants of the enzyme. Students also perform plasmid DNA purification, digestion, and gel analysis. In addition to simply learning important techniques, students acquire novel biochemical data in their kinetic...
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