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Elucidation and control of low and high active populations of alkaline phosphatase molecules for quantitative digital bioassay

6 May 2021

Abstract excerpt

Abstract Alkaline phosphatase (ALP), a homo‐dimeric enzyme has been widely used in various bioassays as disease markers and enzyme probes. Recent advancements of digital bioassay revolutionized ALP‐based diagnostic assays as seen in rapid growth of digital ELISA and the emerging multiplex profiling of single‐molecule ALP isomers. However, the intrinsic heterogeneity found among ALP molecules hampers the ALP‐based...

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PMID: 33955095
DOI: 10.1002/pro.4102

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