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Article

Kinetic analysis of phenylalanine dehydrogenase mutants designed for aliphatic amino acid dehydrogenase activity with guidance from homology-based modelling.

European journal of biochemistry - 1 Dec 2003

Seah Stephen Y K, Britton K Linda, Rice David W, Asano Yasuhisa, Engel Paul C

Abstract excerpt

Through comparison with the high-resolution structure of Clostridium symbiosum glutamate dehydrogenase, the different substrate specificities of the homologous enzymes phenylalanine dehydrogenase and leucine dehydrogenase were attributed to two residues, glycine 124 and leucine 307, in Bacillus sphaericus phenylalanine dehydrogenase, which are replaced with alanine and valine in leucine dehydrogenases. As...

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PMID: 14622249
DOI: 10.1046/j.1432-1033.2003.03852.x

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