Article
Engineering the substrate specificity of Alcaligenes D-aminoacylase useful for the production of D-amino acids by optical resolution.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences - 1 Nov 2011
Yano Shigekazu, Haruta Hiroyuki, Ikeda Takuya, Kikuchi Takeshi, Murakami Masahiro, Moriguchi Mitsuaki, Wakayama Mamoru
Abstract excerpt
D-Aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 (AxD-NAase) offers a novel biotechnological application, the production of D-amino acid from the racemic mixture of N-acyl-DL-amino acids. However, its substrate specificity is biased toward certain N-acyl-D-amino acids. To construct mutant AxD-NAases with substrate specificities different from those of wild-type enzyme, the substrate...
Topics
- Alcaligenes
- Amidohydrolases
- Amino Acids
- Binding Sites
- Circular Dichroism
- Enzyme Stability
- Escherichia coli
- Hydrogen-Ion Concentration
- Kinetics
- Models, Molecular
- Mutagenesis, Site-Directed
- Mutation
- Recombinant Proteins