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Article

Engineering the substrate specificity of Alcaligenes D-aminoacylase useful for the production of D-amino acids by optical resolution.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences - 1 Nov 2011

Yano Shigekazu, Haruta Hiroyuki, Ikeda Takuya, Kikuchi Takeshi, Murakami Masahiro, Moriguchi Mitsuaki, Wakayama Mamoru

Abstract excerpt

D-Aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 (AxD-NAase) offers a novel biotechnological application, the production of D-amino acid from the racemic mixture of N-acyl-DL-amino acids. However, its substrate specificity is biased toward certain N-acyl-D-amino acids. To construct mutant AxD-NAases with substrate specificities different from those of wild-type enzyme, the substrate...

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PMID: 21546325
DOI: 10.1016/j.jchromb.2011.03.030

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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