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Article

Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation.

Genome biology - 17 Jan 2023

Jin Qin, Liu Xiaoyi, Zhuang Zhenpeng, Huang Jiayuan, Gou Shixue, Shi Hui, Zhao Yu, Ouyang Zhen, Liu Zhaoming, Li Lei, Mao Junjie, Ge Weikai, Chen Fangbing, Yu Manya, Guan Yezhi, Ye Yinghua, Tang Chengcheng, Huang Ren, Wang Kepin, Lai Liangxue

Abstract excerpt

BACKGROUND: CRISPR-based toolkits have dramatically increased the ease of genome and epigenome editing. SpCas9 is the most widely used nuclease. However, the difficulty of delivering SpCas9 and inability to modulate its expression in vivo hinder its widespread adoption in large animals. RESULTS: Here, to circumvent these obstacles, a doxycycline-inducible SpCas9-expressing (DIC) pig model was generated by precise...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 36650523
DOI: 10.1186/s13059-023-02851-x
PMCID: PMC9843877

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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