Article
Integrated Single-Cell (Phospho-)Protein and RNA Detection Uncovers Phenotypic Characteristics and Active Signal Transduction of Human Antibody-Secreting Cells.
Molecular & cellular proteomics : MCP - 1 Feb 2023
van Buijtenen Erik, Janssen Wout, Vink Paul, Habraken Maurice J M, Wingens Laura J A, van Elsas Andrea, Huck Wilhelm T S, van Buggenum Jessie A G L, van Eenennaam Hans
Abstract excerpt
Single-cell technologies are currently widely applied to obtain a deeper understanding of the phenotype of single-cells in heterogenous mixtures. However, integrated multilayer approaches including simultaneous detection of mRNA, protein expression, and intracellular phospho-proteins are still challenging. Here, we combined an adapted method to in vitro-differentiate peripheral B-cells into antibody-secreting...
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