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Article

Non-homologous end-joining-deficient filamentous fungal strains mitigate the impact of off-target mutations during the application of CRISPR/Cas9.

mBio - 31 Aug 2023

Garrigues Sandra, Peng Mao, Kun Roland S, de Vries Ronald P

Abstract excerpt

CRISPR/Cas9 genome editing technology has been implemented in almost all living organisms. Its editing precision appears to be very high and therefore could represent a big change from conventional genetic engineering approaches. However, guide RNA binding to nucleotides similar to the target site could result in undesired off-target mutations. Despite this, evaluating whether mutations occur is rarely performed...

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PMID: 37486124
DOI: 10.1128/mbio.00668-23
PMCID: PMC10470509

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