Article
On- and Off-Target Analyses of CRISPR-Cas12b Genome Editing Systems in Rice.
The CRISPR journal - 1 Feb 2023
Gurel Filiz, Wu Yuechao, Pan Changtian, Cheng Yanhao, Li Gen, Zhang Tao, Qi Yiping
Abstract excerpt
The CRISPR-associated Cas12b system is the third most efficient CRISPR tool for targeted genome editing in plants after Cas9 and Cas12a. Although the genome editing ability of AaCas12b has been previously investigated in rice, its off-target effects in plants are largely not known. In this study, we first engineered single-guide RNA (sgRNA) complexes with various RNA scaffolds to enhance editing frequency. We...
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