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A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 (Cas12a) nucleases in rice.

Genome biology - 4 Jul 2018

Tang Xu, Liu Guanqing, Zhou Jianping, Ren Qiurong, You Qi, Tian Li, Xin Xuhui, Zhong Zhaohui, Liu Binglin, Zheng Xuelian, Zhang Dengwei, Malzahn Aimee, Gong Zhiyun, Qi Yiping, Zhang Tao, Zhang Yong

Abstract excerpt

BACKGROUND: Targeting specificity has been a barrier to applying genome editing systems in functional genomics, precise medicine and plant breeding. In plants, only limited studies have used whole-genome sequencing (WGS) to test off-target effects of Cas9. The cause of numerous discovered mutations is still controversial. Furthermore, WGS-based off-target analysis of Cpf1 (Cas12a) has not been reported in any...

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Identifiers

PMID: 29973285
DOI: 10.1186/s13059-018-1458-5
PMCID: PMC6031188

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Source receipt: pubmed, retrieved 7 Sept 2026.

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