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Article

Development, Evaluation, and Implementation of a House-Made Targeted Next-Generation Sequencing Spoligotyping in a French Laboratory.

International journal of molecular sciences - 25 Sept 2022

Genestet Charlotte, Baffert Yannick, Vallée Maxime, Bernard Albin, Benito Yvonne, Lina Gérard, Hodille Elisabeth, Dumitrescu Oana

Abstract excerpt

Epidemiological studies investigating transmission chains of tuberculosis are undertaken worldwide to tackle its spread. CRISPR locus diversity, called spoligotyping, is a widely used genotyping assay for Mycobacterium tuberculosis complex (MTBC) characterization. Herein, we developed a house-made targeted next-generation sequencing (tNGS) spoligotyping, and compared its outputs with those of membrane-based...

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Identifiers

PMID: 36232601
DOI: 10.3390/ijms231911302
PMCID: PMC9569608

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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