Article
The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: providing guidelines for Quality Assurance when working on membranes.
BMC infectious diseases - 28 Apr 2011
Abadia Edgar, Zhang Jian, Ritacco Viviana, Kremer Kristin, Ruimy Raymond, Rigouts Leen, Gomes Harrison Magdinier, Elias Atiná Ribeiro, Fauville-Dufaux Maryse, Stoffels Karolien, Rasolofo-Razanamparany Voahangy, Garcia de Viedma Darío, Herranz Marta, Al-Hajoj Sahal, Rastogi Nalin, Garzelli Carlo, Tortoli Enrico, Suffys Philip N, van Soolingen Dick, Refrégier Guislaine, Sola Christophe
Abstract excerpt
BACKGROUND: The classical spoligotyping technique, relying on membrane reverse line-blot hybridization of the spacers of the Mycobacterium tuberculosis CRISPR locus, is used world-wide (598 references in Pubmed on April 8th, 2011). However, until now no inter-laboratory quality control study had been undertaken to validate this technique. We analyzed the quality of membrane-based spoligotyping by comparing it to...
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