Article
Microbead-based spoligotyping of Mycobacterium tuberculosis from Ziehl-Neelsen-stained microscopy preparations in Ethiopia.
Scientific reports - 5 Mar 2018
Molina-Moya Barbara, Agonafir Mulualem, Blanco Silvia, Dacombe Russell, Gomgnimbou Michel K, Spinasse Lizania, Gomes-Fernandes Meissiner, Datiko Daniel G, Edwards Thomas, Cuevas Luis E, Dominguez Jose, Sola Christophe
Abstract excerpt
The worldwide dissemination of Mycobacterium tuberculosis strains has led to the study of their genetic diversity. One of the most used genotyping methods is spoligotyping, based on the detection of spacers in the clustered regularly interspaced short palindromic repeats (CRISPR) locus. This study assessed the performance of a microbead-based spoligotyping assay using samples extracted from Ziehl-Neelsen-stained...
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