Article
Robust linear DNA degradation supports replication-initiation-defective mutants in Escherichia coli.
G3 (Bethesda, Md.) - 4 Nov 2022
Rao T V Pritha, Kuzminov Andrei
Abstract excerpt
RecBCD helicase/nuclease supports replication fork progress via recombinational repair or linear DNA degradation, explaining recBC mutant synthetic lethality with replication elongation defects. Since replication initiation defects leave chromosomes without replication forks, these should be insensitive to the recBCD status. Surprisingly, we found that both Escherichia coli dnaA46(Ts) and dnaC2(Ts) initiation...
Topics
- Escherichia coli
- Escherichia coli Proteins
- DNA Replication
- DNA Helicases
- DNA
- Mutant Proteins
- DNA, Bacterial
- Mutation
- Bacterial Proteins
- Chromosomes, Bacterial