Article
Clock genes rescue nphp mutations in zebrafish.
Human molecular genetics - 16 Dec 2022
Kayser Nicolas, Zaiser Friedemann, Veenstra Anna C, Wang Hui, Göcmen Burulca, Eckert Priska, Franz Henriette, Köttgen Anna, Walz Gerd, Yakulov Toma A
Abstract excerpt
The zebrafish pronephros model, using morpholino oligonucleotides (MO) to deplete target genes, has been extensively used to characterize human ciliopathy phenotypes. Recently, discrepancies between MO and genetically defined mutants have questioned this approach. We analyzed zebrafish with mutations in the nphp1-4-8 module to determine the validity of MO-based results. While MO-mediated depletion resulted in...
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