Optimizing bulk segregant analysis of drug resistance using Plasmodium falciparum genetic crosses conducted in humanized mice

Abstract excerpt

Classical malaria parasite genetic crosses involve isolation, genotyping, and phenotyping of progeny parasites, which is time consuming and laborious. We tested a rapid alternative approach—bulk segregant analysis (BSA)—that utilizes sequencing of bulk progeny populations with and without drug selection for rapid identification of drug resistance loci. We used dihydroartemisinin (DHA) selection in two genetic...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Malaria Research and Control
• Share to Mosquito-borne diseases and control
• Share to Trypanosoma species research and implications