Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

CRISPR/Cas13-assisted hepatitis B virus covalently closed circular DNA detection

17 Mar 2022

Abstract excerpt

BACKGROUND AND AIMS: The formation of an intranuclear pool of covalently closed circular DNA (cccDNA) in the liver is the main cause of persistent hepatitis B virus (HBV) infection. Here, we established highly sensitive and specific methods to detect cccDNA based on CRISPR-Cas13a technology. METHODS: We used plasmid-safe ATP-dependent DNase (PSAD) enzymes and HindIII to digest loose circle rcDNA and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Advanced biosensing and bioanalysis techniques
Share to CRISPR and Genetic Engineering
Share to Hepatitis B Virus Studies

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 35298777
DOI: 10.1007/s12072-022-10311-0

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

CRISPR/Cas13-assisted hepatitis B virus covalently closed circular DNA detectionPMID 35298777Read ↗

Complete Spectrum of CRISPR/Cas9-induced Mutations on HBV cccDNA.Semantic proximity Read ↗

Application of CRISPR/Cas9 Technology to HBVSemantic proximity Read ↗

Highly sensitive and specific detection of hepatitis B virus DNA and drug resistance mutations utilizing the PCR-based CRISPR-Cas13a system.Semantic proximity Read ↗

A CRISPR-Cas12b–Based Platform for Ultrasensitive, Rapid, and Highly Specific Detection of Hepatitis B Virus Genotypes B and C in Clinical ApplicationSemantic proximity Read ↗

CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virusSemantic proximity Read ↗

CRISPR/Cas9 for hepatitis B virus infection treatmentSemantic proximity Read ↗

CRISPR/Cas13a-Assisted accurate and portable hepatitis D virus RNA detectionSemantic proximity Read ↗

Recent Progress and Future Prospective in HBV Cure by CRISPR/CasSemantic proximity Read ↗

CRISPR-Cas9 Targeting of Hepatitis B Virus Covalently Closed Circular DNA Generates Transcriptionally Active Episomal VariantsSemantic proximity Read ↗

CRISPR/Cas and Hepatitis B Therapy: Technological Advances and Practical BarriersSemantic proximity Read ↗

CRISPR/Cas9 nickase-mediated disruption of hepatitis B virus open reading frame S and XSemantic proximity Read ↗

Potential of CRISPR/Cas system as emerging tools in the detection of viral hepatitis infectionSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.