Article
Nitrogen Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing.
Applied and environmental microbiology - 12 Apr 2022
Schmidt Matthias, Pearson Allison N, Incha Matthew R, Thompson Mitchell G, Baidoo Edward E K, Kakumanu Ramu, Mukhopadhyay Aindrila, Shih Patrick M, Deutschbauer Adam M, Blank Lars M, Keasling Jay D
Abstract excerpt
Pseudomonas putida KT2440 has long been studied for its diverse and robust metabolisms, yet many genes and proteins imparting these growth capacities remain uncharacterized. Using pooled mutant fitness assays, we identified genes and proteins involved in the assimilation of 52 different nitrogen containing compounds. To assay amino acid biosynthesis, 19 amino acid drop-out conditions were also tested. From these...
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