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Article

Whole genome analysis for 163 gRNAs in Cas9-edited mice reveals minimal off-target activity.

Communications biology - 10 Jun 2023

Peterson Kevin A, Khalouei Sam, Hanafi Nour, Wood Joshua A, Lanza Denise G, Lintott Lauri G, Willis Brandon J, Seavitt John R, Braun Robert E, Dickinson Mary E, White Jacqueline K, Lloyd K C Kent, Heaney Jason D, Murray Stephen A, Ramani Arun, Nutter Lauryl M J

Abstract excerpt

Genome editing with CRISPR-associated (Cas) proteins holds exceptional promise for "correcting" variants causing genetic disease. To realize this promise, off-target genomic changes cannot occur during the editing process. Here, we use whole genome sequencing to compare the genomes of 50 Cas9-edited founder mice to 28 untreated control mice to assess the occurrence of S. pyogenes Cas9-induced off-target...

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Identifiers

PMID: 37301944
DOI: 10.1038/s42003-023-04974-0
PMCID: PMC10257658

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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