Article
Construction of a deletion library using a mixture of 5'-truncated primers for inverse PCR (IPCR).
Nucleic acids research - 15 Mar 1997
Pues H, Holz B, Weinhold E
Abstract excerpt
A quick in vitro mutagenesis method for the construction of nested deletion libraries was developed. Many deletions can be obtained in a single inverse PCR (IPCR) by replacing one of the two primers with a mixture of 5'-truncated oligodeoxynucleotides. Since chemical DNA synthesis proceeds from t...
Topics
- Base Sequence
- DNA
- DNA Primers
- Deoxyribonucleases, Type II Site-Specific
- Gene Library
- Genetic Variation
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
- Protein Engineering
- Regulatory Sequences, Nucleic Acid
- Sequence Deletion
- Thermus