Construction of a deletion library using a mixture of 5'-truncated primers for inverse PCR (IPCR).

doi:10.1093/nar/25.6.1303

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Construction of a deletion library using a mixture of 5'-truncated primers for inverse PCR (IPCR).

Nucleic acids research - 15 Mar 1997

Pues H, Holz B, Weinhold E

Abstract excerpt

A quick in vitro mutagenesis method for the construction of nested deletion libraries was developed. Many deletions can be obtained in a single inverse PCR (IPCR) by replacing one of the two primers with a mixture of 5'-truncated oligodeoxynucleotides. Since chemical DNA synthesis proceeds from the 3'to the 5'end, such a mixture of 5'-truncated oligodeoxynucleotides can easily be obtained in a single automated...

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Construction of a deletion library using a mixture of 5'-truncated primers for inverse PCR (IPCR).

Abstract excerpt

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Construction of a deletion library using a mixture of 5'-truncated primers for inverse PCR (IPCR).

Abstract excerpt

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