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Article

Probing the mechanisms of two exonuclease domain mutators of DNA polymerase ϵ.

Nucleic acids research - 25 Jan 2022

Dahl Joseph M, Thomas Natalie, Tracy Maxwell A, Hearn Brady L, Perera Lalith, Kennedy Scott R, Herr Alan J, Kunkel Thomas A

Abstract excerpt

We report the properties of two mutations in the exonuclease domain of the Saccharomyces cerevisiae DNA polymerase ϵ. One, pol2-Y473F, increases the mutation rate by about 20-fold, similar to the catalytically dead pol2-D290A/E290A mutant. The other, pol2-N378K, is a stronger mutator. Both retain the ability to excise a nucleotide from double-stranded DNA, but with impaired activity. pol2-Y473F degrades DNA...

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Identifiers

PMID: 35037018
DOI: 10.1093/nar/gkab1255
PMCID: PMC8789060

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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