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Recombinant production of active microbial transglutaminase in E. coli by using self-cleavable zymogen with mutated propeptide.

Protein expression and purification - 1 Dec 2020

Sato Ryo, Minamihata Kosuke, Ariyoshi Ryutaro, Taniguchi Hiromasa, Kamiya Noriho

Abstract excerpt

Microbial transglutaminase from Streptomyces mobaraensis (MTG) has been widely used in food industry and also in research and medical applications, since it can site-specifically modify proteins by the cross-linking reaction of glutamine residue and the primary amino group. The recombinant expression system of MTG in E. coli provides better accessibility for the researchers and thus can promote further...

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PMID: 32827662
DOI: 10.1016/j.pep.2020.105730

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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