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Article

Constitutive expression of active microbial transglutaminase in Escherichia coli and comparative characterization to a known variant.

BMC biotechnology - 28 Feb 2017

Javitt Gabe, Ben-Barak-Zelas Zohar, Jerabek-Willemsen Moran, Fishman Ayelet

Abstract excerpt

BACKGROUND: Microbial transglutaminase (mTG) is a robust enzyme catalyzing the formation of an isopeptide bond between glutamine and lysine residues. It has found use in food applications, pharmaceuticals, textiles, and biomedicine. Overexpression of soluble and active mTG in E. coli has been limited due to improper protein folding and requirement for proteolytic cleavage of the pro-domain. Furthermore, to...

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Identifiers

PMID: 28245818
DOI: 10.1186/s12896-017-0339-4
PMCID: PMC5331659

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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