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Article

Conditional CRISPR-Cas Genome Editing in Drosophila to Generate Intestinal Tumors.

Cells - 13 Nov 2021

Bahuguna Shivohum, Redhai Siamak, Zhou Jun, Wang Tianyu, Port Fillip, Boutros Michael

Abstract excerpt

CRISPR-Cas has revolutionized genetics and extensive efforts have been made to enhance its editing efficiency by developing increasingly more elaborate tools. Here, we evaluate the CRISPR-Cas9 system in Drosophila melanogaster to assess its ability to induce stem cell-derived tumors in the intestine. We generated conditional tissue-specific CRISPR knockouts using different Cas9 expression vectors with guide RNAs...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 34831379
DOI: 10.3390/cells10113156
PMCID: PMC8620722

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